Partial Lack of N-Acetyl Substitution of Glucosamine in the Peptidoglycan of the Budding Phototrophic Rhodomicrobium vannielii
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چکیده
The rigid layer and peptidoglycan fractions from two strains (ATCC 17100 and Rm 5) of the budding phototrophic Rhodomicrobium vannielii were isolated. Rigid layers of both strains con tain protein in addition to peptidoglycan. They were free of polysaccharides and fatty acids. The respective peptidoglycan fractions contain glucosamine, muramic acid, l and D-alanine, D-glutamic and m eso-diaminopimelic acid in approximately equimolar ratios except for a signifi cant lower relative D-alanine content. Analysis of partial acid hydrolysates revealed A 1 '/-type structure of Rhodomicrobium vannielii peptidoglycan (shown with strain ATCC 17100). An about 10—30% lack o f N-acetylation of glucosamine was indicated. The degree of cross-linkage was found to be about 60%. No differences in peptidoglycan com position and degree of cross-linkage were found between swarmerand chain-cells as examined with strain Rm5.
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تاریخ انتشار 2013